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cell suspension  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cell suspension
    Cell Suspension, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 2096 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+suspension/Neural+Tissue+Dissociation+Kit/pm42844257-299-19-34
    Average 97 stars, based on 2096 article reviews
    cell suspension - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Sterility:

    Article Title: Activation of Angiotensin Converting Enzyme 2 (ACE2) Mitigates Gastrointestinal Acute Radiation Syndrome (GI-ARS).
    Article Snippet: .. Jejunums were gently dissected, rinsed with sterile saline, and dissociated to a single cell suspension using the gentleMACS Dissociator (Miltenyi) and the human tumor dissociation kit (Miltenyi, 130-095-929). ..

    Saline:

    Article Title: Activation of Angiotensin Converting Enzyme 2 (ACE2) Mitigates Gastrointestinal Acute Radiation Syndrome (GI-ARS).
    Article Snippet: .. Jejunums were gently dissected, rinsed with sterile saline, and dissociated to a single cell suspension using the gentleMACS Dissociator (Miltenyi) and the human tumor dissociation kit (Miltenyi, 130-095-929). ..

    Single Cell:

    Article Title: Activation of Angiotensin Converting Enzyme 2 (ACE2) Mitigates Gastrointestinal Acute Radiation Syndrome (GI-ARS).
    Article Snippet: .. Jejunums were gently dissected, rinsed with sterile saline, and dissociated to a single cell suspension using the gentleMACS Dissociator (Miltenyi) and the human tumor dissociation kit (Miltenyi, 130-095-929). ..

    Article Title: Chemotherapy reshapes cellular clonal landscape via persister programs in breast cancer xenografts
    Article Snippet: .. PDTX fragments were dissociated into a single cell suspension (Miltenyi Biotec, Cat. no. 130-095-929) and depleted of mouse cells (Miltenyi Biotec, Cat. no. 130-104-694). ..

    Article Title: Periosteal mitochondria DNA structures drive aging-associated poor skeletal repair.
    Article Snippet: Then, fibrous callus was digested in 1 mL pre-warmed digestion solution (3 mg/mL collagenase I (Sigma, USA) in αMEM) for 45 min in a water bath at 37 °C with gentle agitation every 10min. .. Then, the mixture of digested minced bones and target cells were treated with red blood cell lysate on ice for 3 min. After lysis, single-cell suspension was sorted using CD45 MicroBeads (Milteny, #130-052-301, Germany) and CD104a (Pdgfra) MicroBeads Kit (Milteny, #130-101-502, Germany). ..

    Article Title: Periosteal mitochondria DNA structures drive aging-associated poor skeletal repair
    Article Snippet: .. Then, fibrous callus was digested in 1 mL pre-warmed digestion solution (3 mg/mL collagenase I (Sigma, USA) in αMEM) for 45 min in a water bath at 37 °C with gentle agitation every 10 min. Then, the mixture of digested minced bones and target cells were treated with red blood cell lysate on ice for 3 min. After lysis, single-cell suspension was sorted using CD45 MicroBeads (Milteny, #130-052-301, Germany) and CD104a (Pdgfra) MicroBeads Kit (Milteny, #130-101-502, Germany). ..

    Article Title: An unbiased whole-genome open reading frame overexpression screen identifies B3GALT2, a novel inducer of cellular ASO activity
    Article Snippet: .. On day 11 273 (replicate 1), 12 (replicate 2) and 13 (replicate 3) cells have been treated with 25 nM SS-ASO for 72 h. After 72 h, 274 cells were dissociated from plates using Accutase (StemCell Technologies, Catalog No. AT104) and filtered through 275 30 μm filters to create a single-cell suspension (Miltenyi Biotec, Catalog No. 130-098-458). .. Next, 38 million cells were 276 centrifuged (610x g, 10 min), supernatant removed, and cell pellets snap frozen; the rest of the cells were prepared 277 in DMEM (phenol free, Thermo Fisher, Catalog No. 21063045) media for sorting.

    Article Title: An unbiased whole-genome open reading frame overexpression screen identifies B3GALT2, a novel inducer of cellular ASO activity
    Article Snippet: .. On days 11 (replicate 1), 12 (replicate 2), and 13 (replicate 3) cells were treated with 25 nM SS-ASO for 72 h. After 72 h, cells were dissociated from plates using Accutase (StemCell Technologies, Catalog No. AT104) and filtered through 30-μm filters to create a single-cell suspension (Miltenyi Biotec, Catalog No. 130-098-458). .. Next, 38 million cells were centrifuged (610 × g , 10 min), supernatant removed, and cell pellets snap frozen; the rest of the cells were prepared in DMEM (phenol free, Thermo Fisher, Catalog No. 21063045) media for sorting.

    Article Title: A BcfA-containing intranasal vaccine generated T H 17 immunity and reduced B. bronchiseptica colonization and disease in mice
    Article Snippet: .. Lungs and nasal septum were isolated and enzymatically digested into a single-cell suspension (Miltenyi Biotec, cat. # 130-095-927), followed by filtration through a 40-μm filter and RBC lysis with ACK lysis buffer. .. Cells were resuspended in T-cell media (RPMI, 10% FBS, 10 μg/ml gentamycin, 5 × 10 −5 M 2-mercaptoethanol) and stimulated with PMA (500 ng/ml)/ionomycin (500 ng/ml) plus protein transport inhibitor (eBioscience, cat. # 00-4975-93) or only T-cell media as a negative control for 5–6 h at 37 °C in a 5% CO 2 incubator.

    Article Title: Targeting Myc activates a tissue-specific tumour resolution programme
    Article Snippet: For single cell RNA sequencing, lungs were isolated from KM mice, placed into MACS tissue storage solution (130-100-008, Miltenyi) on ice and processed to single cell suspension on a gentleMACS Octo Dissociator with Heaters (130-096-427, Miltenyi) according to manufacturers’ instructions. .. In brief, after manually dissecting the lungs in ∼1mm pieces, they were further processed into a single cell suspension using the mouse tumour dissociation kit (130-096-730, Miltenyi) in gentleMACS C tubes (130-096-334, Miltenyi). .. After dissociation, the cell suspension was incubated with Red Blood Lysis Solution (130-094-183, Miltenyi) to remove red blood cells.

    Suspension:

    Article Title: Activation of Angiotensin Converting Enzyme 2 (ACE2) Mitigates Gastrointestinal Acute Radiation Syndrome (GI-ARS).
    Article Snippet: .. Jejunums were gently dissected, rinsed with sterile saline, and dissociated to a single cell suspension using the gentleMACS Dissociator (Miltenyi) and the human tumor dissociation kit (Miltenyi, 130-095-929). ..

    Article Title: Chemotherapy reshapes cellular clonal landscape via persister programs in breast cancer xenografts
    Article Snippet: .. PDTX fragments were dissociated into a single cell suspension (Miltenyi Biotec, Cat. no. 130-095-929) and depleted of mouse cells (Miltenyi Biotec, Cat. no. 130-104-694). ..

    Article Title: Periosteal mitochondria DNA structures drive aging-associated poor skeletal repair.
    Article Snippet: Then, fibrous callus was digested in 1 mL pre-warmed digestion solution (3 mg/mL collagenase I (Sigma, USA) in αMEM) for 45 min in a water bath at 37 °C with gentle agitation every 10min. .. Then, the mixture of digested minced bones and target cells were treated with red blood cell lysate on ice for 3 min. After lysis, single-cell suspension was sorted using CD45 MicroBeads (Milteny, #130-052-301, Germany) and CD104a (Pdgfra) MicroBeads Kit (Milteny, #130-101-502, Germany). ..

    Article Title: Periosteal mitochondria DNA structures drive aging-associated poor skeletal repair
    Article Snippet: .. Then, fibrous callus was digested in 1 mL pre-warmed digestion solution (3 mg/mL collagenase I (Sigma, USA) in αMEM) for 45 min in a water bath at 37 °C with gentle agitation every 10 min. Then, the mixture of digested minced bones and target cells were treated with red blood cell lysate on ice for 3 min. After lysis, single-cell suspension was sorted using CD45 MicroBeads (Milteny, #130-052-301, Germany) and CD104a (Pdgfra) MicroBeads Kit (Milteny, #130-101-502, Germany). ..

    Article Title: An unbiased whole-genome open reading frame overexpression screen identifies B3GALT2, a novel inducer of cellular ASO activity
    Article Snippet: .. On day 11 273 (replicate 1), 12 (replicate 2) and 13 (replicate 3) cells have been treated with 25 nM SS-ASO for 72 h. After 72 h, 274 cells were dissociated from plates using Accutase (StemCell Technologies, Catalog No. AT104) and filtered through 275 30 μm filters to create a single-cell suspension (Miltenyi Biotec, Catalog No. 130-098-458). .. Next, 38 million cells were 276 centrifuged (610x g, 10 min), supernatant removed, and cell pellets snap frozen; the rest of the cells were prepared 277 in DMEM (phenol free, Thermo Fisher, Catalog No. 21063045) media for sorting.

    Article Title: An unbiased whole-genome open reading frame overexpression screen identifies B3GALT2, a novel inducer of cellular ASO activity
    Article Snippet: .. On days 11 (replicate 1), 12 (replicate 2), and 13 (replicate 3) cells were treated with 25 nM SS-ASO for 72 h. After 72 h, cells were dissociated from plates using Accutase (StemCell Technologies, Catalog No. AT104) and filtered through 30-μm filters to create a single-cell suspension (Miltenyi Biotec, Catalog No. 130-098-458). .. Next, 38 million cells were centrifuged (610 × g , 10 min), supernatant removed, and cell pellets snap frozen; the rest of the cells were prepared in DMEM (phenol free, Thermo Fisher, Catalog No. 21063045) media for sorting.

    Article Title: A BcfA-containing intranasal vaccine generated T H 17 immunity and reduced B. bronchiseptica colonization and disease in mice
    Article Snippet: .. Lungs and nasal septum were isolated and enzymatically digested into a single-cell suspension (Miltenyi Biotec, cat. # 130-095-927), followed by filtration through a 40-μm filter and RBC lysis with ACK lysis buffer. .. Cells were resuspended in T-cell media (RPMI, 10% FBS, 10 μg/ml gentamycin, 5 × 10 −5 M 2-mercaptoethanol) and stimulated with PMA (500 ng/ml)/ionomycin (500 ng/ml) plus protein transport inhibitor (eBioscience, cat. # 00-4975-93) or only T-cell media as a negative control for 5–6 h at 37 °C in a 5% CO 2 incubator.

    Article Title: Targeting Myc activates a tissue-specific tumour resolution programme
    Article Snippet: For single cell RNA sequencing, lungs were isolated from KM mice, placed into MACS tissue storage solution (130-100-008, Miltenyi) on ice and processed to single cell suspension on a gentleMACS Octo Dissociator with Heaters (130-096-427, Miltenyi) according to manufacturers’ instructions. .. In brief, after manually dissecting the lungs in ∼1mm pieces, they were further processed into a single cell suspension using the mouse tumour dissociation kit (130-096-730, Miltenyi) in gentleMACS C tubes (130-096-334, Miltenyi). .. After dissociation, the cell suspension was incubated with Red Blood Lysis Solution (130-094-183, Miltenyi) to remove red blood cells.

    Lysis:

    Article Title: Periosteal mitochondria DNA structures drive aging-associated poor skeletal repair.
    Article Snippet: Then, fibrous callus was digested in 1 mL pre-warmed digestion solution (3 mg/mL collagenase I (Sigma, USA) in αMEM) for 45 min in a water bath at 37 °C with gentle agitation every 10min. .. Then, the mixture of digested minced bones and target cells were treated with red blood cell lysate on ice for 3 min. After lysis, single-cell suspension was sorted using CD45 MicroBeads (Milteny, #130-052-301, Germany) and CD104a (Pdgfra) MicroBeads Kit (Milteny, #130-101-502, Germany). ..

    Article Title: Periosteal mitochondria DNA structures drive aging-associated poor skeletal repair
    Article Snippet: .. Then, fibrous callus was digested in 1 mL pre-warmed digestion solution (3 mg/mL collagenase I (Sigma, USA) in αMEM) for 45 min in a water bath at 37 °C with gentle agitation every 10 min. Then, the mixture of digested minced bones and target cells were treated with red blood cell lysate on ice for 3 min. After lysis, single-cell suspension was sorted using CD45 MicroBeads (Milteny, #130-052-301, Germany) and CD104a (Pdgfra) MicroBeads Kit (Milteny, #130-101-502, Germany). ..

    Article Title: A BcfA-containing intranasal vaccine generated T H 17 immunity and reduced B. bronchiseptica colonization and disease in mice
    Article Snippet: .. Lungs and nasal septum were isolated and enzymatically digested into a single-cell suspension (Miltenyi Biotec, cat. # 130-095-927), followed by filtration through a 40-μm filter and RBC lysis with ACK lysis buffer. .. Cells were resuspended in T-cell media (RPMI, 10% FBS, 10 μg/ml gentamycin, 5 × 10 −5 M 2-mercaptoethanol) and stimulated with PMA (500 ng/ml)/ionomycin (500 ng/ml) plus protein transport inhibitor (eBioscience, cat. # 00-4975-93) or only T-cell media as a negative control for 5–6 h at 37 °C in a 5% CO 2 incubator.

    Gentle:

    Article Title: Periosteal mitochondria DNA structures drive aging-associated poor skeletal repair
    Article Snippet: .. Then, fibrous callus was digested in 1 mL pre-warmed digestion solution (3 mg/mL collagenase I (Sigma, USA) in αMEM) for 45 min in a water bath at 37 °C with gentle agitation every 10 min. Then, the mixture of digested minced bones and target cells were treated with red blood cell lysate on ice for 3 min. After lysis, single-cell suspension was sorted using CD45 MicroBeads (Milteny, #130-052-301, Germany) and CD104a (Pdgfra) MicroBeads Kit (Milteny, #130-101-502, Germany). ..

    Isolation:

    Article Title: A BcfA-containing intranasal vaccine generated T H 17 immunity and reduced B. bronchiseptica colonization and disease in mice
    Article Snippet: .. Lungs and nasal septum were isolated and enzymatically digested into a single-cell suspension (Miltenyi Biotec, cat. # 130-095-927), followed by filtration through a 40-μm filter and RBC lysis with ACK lysis buffer. .. Cells were resuspended in T-cell media (RPMI, 10% FBS, 10 μg/ml gentamycin, 5 × 10 −5 M 2-mercaptoethanol) and stimulated with PMA (500 ng/ml)/ionomycin (500 ng/ml) plus protein transport inhibitor (eBioscience, cat. # 00-4975-93) or only T-cell media as a negative control for 5–6 h at 37 °C in a 5% CO 2 incubator.

    Filtration:

    Article Title: A BcfA-containing intranasal vaccine generated T H 17 immunity and reduced B. bronchiseptica colonization and disease in mice
    Article Snippet: .. Lungs and nasal septum were isolated and enzymatically digested into a single-cell suspension (Miltenyi Biotec, cat. # 130-095-927), followed by filtration through a 40-μm filter and RBC lysis with ACK lysis buffer. .. Cells were resuspended in T-cell media (RPMI, 10% FBS, 10 μg/ml gentamycin, 5 × 10 −5 M 2-mercaptoethanol) and stimulated with PMA (500 ng/ml)/ionomycin (500 ng/ml) plus protein transport inhibitor (eBioscience, cat. # 00-4975-93) or only T-cell media as a negative control for 5–6 h at 37 °C in a 5% CO 2 incubator.

    Red Blood Cell Lysis:

    Article Title: A BcfA-containing intranasal vaccine generated T H 17 immunity and reduced B. bronchiseptica colonization and disease in mice
    Article Snippet: .. Lungs and nasal septum were isolated and enzymatically digested into a single-cell suspension (Miltenyi Biotec, cat. # 130-095-927), followed by filtration through a 40-μm filter and RBC lysis with ACK lysis buffer. .. Cells were resuspended in T-cell media (RPMI, 10% FBS, 10 μg/ml gentamycin, 5 × 10 −5 M 2-mercaptoethanol) and stimulated with PMA (500 ng/ml)/ionomycin (500 ng/ml) plus protein transport inhibitor (eBioscience, cat. # 00-4975-93) or only T-cell media as a negative control for 5–6 h at 37 °C in a 5% CO 2 incubator.



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